Restriction Digest

Goal:
Model the process of producing recombinant DNA.
Describe the characteristics of plasmids and explain how plasmids are used in cloning a gene.
Describe the function of restriction enzymes and explain how to use restriction enzymes to create a recombinant DNA.

Agenda:
Clone That Gene!
Lab
Summarize

Clone That Gene!
Read and Follow the directions at the end of this chapter.

Lab
Today we will be performing a restriction digest to cut up plasmids so we can make a recombinant plasmid with the rfp gene.

• You will work in teams of 4.
• Follow the lab procedure VERY CAREFULLY. We don't have extra if you mess up. 
• Make sure you use the micropipets properly (remember, 1st stop/2nd stop).

Summarize
On the restriction digest slide in your competency do the following.
Left Side:

1. Define plasmids and restriction enzymes
2. Use the key to explain what each of the beads represent and why they are important. Refer back to the reading (pg. 46)

Right Side:

1. In your petri dish you have the 2 plasmids and 2 restriction enzymes that we worked with today. Work with your elbow partner to create an image for what is in each of the 4 tubes (K+, K-, A+, A-)