Genetic Engineering Competency Catch-Up

Goal:
Describe the role of DNA ligase in replication and explain how DNA ligase is used to create a recombinant DNA.
Describe the possible recombinant plasmids that form when ligating a restriction digest.

Agenda:
Final Teamwork Evaluation
Finish Restriction Digest Slide
Ligation Prediction
Work on Slides

Final Teamwork Evaluation
Follow the Feedback Protocol. Scribe, use the BLUE highlighter to mark the final score for each team member.

Finish Restriction Digest Slide
Left Side:
Define plasmids and restriction enzymes
Use the key to explain what each of the beads represent and why they are important. Refer back to the reading (pg. 46 of reading)
Right Side:
In your petri dish you have the 2 plasmids and 2 restriction enzymes that we worked with today. Work with your elbow partner to create an image for what is in each of the 4 tubes (K+, K-, A+, A-)

Ligation Prediction
Left Side:
Define ligase and recombinant DNA.
Which recombinant plasmid will produce RFP? How do you know that?
Right Side:
Use the model to show all possible ligation products that could be present in the LIG tube. Also show what is in the A+ and K+ tubes.

Work on Slides
By now you should have finished 3 slides: Intro, Restriction Digest, and Ligation