Genetic Engineering Competency Catch-Up

Goal:
Describe the role of DNA ligase in replication and explain how DNA ligase is used to create a recombinant DNA.
Describe the possible recombinant plasmids that form when ligating a restriction digest.

Agenda:
Final Teamwork Evaluation
Finish Restriction Digest Slide
Ligation Prediction
Work on Slides

Final Teamwork Evaluation
Follow the Feedback Protocol. Scribe, use the BLUE highlighter to mark the final score for each team member.

Finish Restriction Digest Slide
Left Side:
Define plasmids and restriction enzymes
Use the key to explain what each of the beads represent and why they are important. Refer back to the reading (pg. 46 of reading)
Right Side:
In your petri dish you have the 2 plasmids and 2 restriction enzymes that we worked with today. Work with your elbow partner to create an image for what is in each of the 4 tubes (K+, K-, A+, A-)

Ligation Prediction
Left Side:
Define ligase and recombinant DNA.
Which recombinant plasmid will produce RFP? How do you know that?
Right Side:
Use the model to show all possible ligation products that could be present in the LIG tube. Also show what is in the A+ and K+ tubes.

Work on Slides
By now you should have finished 3 slides: Intro, Restriction Digest, and Ligation

Restriction Digest

Goal:
Model the process of producing recombinant DNA.
Describe the characteristics of plasmids and explain how plasmids are used in cloning a gene.
Describe the function of restriction enzymes and explain how to use restriction enzymes to create a recombinant DNA.

Agenda:
Clone That Gene!
Lab
Summarize

Clone That Gene!
Read and Follow the directions at the end of this chapter.

Lab
Today we will be performing a restriction digest to cut up plasmids so we can make a recombinant plasmid with the rfp gene.

• You will work in teams of 4.
• Follow the lab procedure VERY CAREFULLY. We don't have extra if you mess up. 
• Make sure you use the micropipets properly (remember, 1st stop/2nd stop).

Summarize
On the restriction digest slide in your competency do the following.
Left Side:

1. Define plasmids and restriction enzymes
2. Use the key to explain what each of the beads represent and why they are important. Refer back to the reading (pg. 46)

Right Side:

1. In your petri dish you have the 2 plasmids and 2 restriction enzymes that we worked with today. Work with your elbow partner to create an image for what is in each of the 4 tubes (K+, K-, A+, A-)

Finish Stop Motion

Goal:
Model the process of producing recombinant DNA.
Describe the characteristics of plasmids and explain how plasmids are used in cloning a gene.
Describe the function of restriction enzymes and explain how to use restriction enzymes to create a recombinant DNA.

Agenda:
Vocab Quiz
Finish Stop Motion
Clone That Gene!

Vocab Quiz
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Finish Stop Motion
Make sure to include the protein purification step.
Once you're done, I will show you how to link it to your Google Slides Competency.

Clone That Gene!
Read and Follow the directions at the end of this chapter.

Genetic Engineering Process

Goal:
Describe the role of biological tools, such as plasmids, restriction enzymes, e.coli, and ligase, in the genetic engineering process.

Agenda:
Warmup
Overview Genetic Engineering Process
Stop Motion

Warmup
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Overview Genetic Engineering Process
Link to Slides

Stop Motion
Make a Stop Motion video animating the ENTIRE genetic engineering process. Include the following vocabulary:
Bacteria
Plasmid
Restriction Enzyme
Ligase Enzyme
Human/Animal Inserted DNA (insulin or red protein??....anything you want)
Recombinant DNA
Transformed Bacteria
Protein (insulin,rfp)

Vocab Quiz Tomorrow

Study Quizlet

What is Genetic Engineering?

Goals:
-Provide examples of how genetic engineering can be used to solve medical problems.
-Explain how genetic engineering creates genetically modified organisms that make human proteins from human DNA.

Agenda:
Warmup
Listen
Read
Write

Warmup
Did you find anything interesting in your family health history that might inform how you approach your health care in the future? If you would like, share on this Padlet.

Considering what we reviewed yesterday, what may be causing these genetic diseases?

Listen
CRISPR Radiolab. How could this technology be used to permanently cure genetic diseases?

Read
"What is genetic engineering?" Use the reading to compare and contrast CRISPR and recombinant DNA technology using a venn diagram.

Write
I shared a Google Slides with you. Your competency is to complete these slides. On the intro slide use what you have learned to answer the questions below in complete sentences.

1. Explain the cause of genetic diseases such as Type 1 Diabetes or Breast Cancer. 
2. How could CRISPR be used to provide a permanent cure for genetic diseases?
3. In recombinant DNA technology, scientists modify a bacterial plasmid by adding a human gene in order to produce human proteins. How does this process help in addressing genetic diseases (for example, with insulin)?
4. In our Biotechnology lab sequence how will what we do be different than making insulin?